[1]周红,邵征洋,连俊兰.脾虚肝旺型功能性消化不良幼鼠模型的建立与评价[J].中医儿科杂志,2017,(04):17-20.[doi:10.16840/j.issn1673-4297.2017.04.05]
 ZHOU Hong,SHAO Zhengyang,LIAN Junlan.Establishment and evaluation of the model of functional dyspepsia young rats in spleen-deficiency and liver-hyperaction[J].Journal of Pediatrics of Traditional Chinese Medicine,2017,(04):17-20.[doi:10.16840/j.issn1673-4297.2017.04.05]
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脾虚肝旺型功能性消化不良幼鼠模型的建立与评价
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《中医儿科杂志》[ISSN:/CN:62-1176/R]

卷:
期数:
2017年04期
页码:
17-20
栏目:
实验研究
出版日期:
2017-07-25

文章信息/Info

Title:
Establishment and evaluation of the model of functional dyspepsia young rats in spleen-deficiency and liver-hyperaction
作者:
周红1 邵征洋2 连俊兰2
1. 浙江萧山医院儿科, 浙江 杭州 311202;
2. 浙江省中西医结合医院儿科, 浙江 杭州 310003
Author(s):
ZHOU Hong1 SHAO Zhengyang2 LIAN Junlan2
1. Department of Pediatrics, Zhejiang Xiaoshan Hospital, Hangzhou, Zhejiang, 311202, China;
2. Department of Pediatrics, Zhejiang Hospital of Integrated Traditional Chinese and Western Medicine, Hangzhou, Zhejiang, 310003, China
关键词:
脾虚肝旺功能性消化不良幼鼠模型
Keywords:
spleen deficiency and liver sufficiencyfunctional dyspepsiayoung ratsmodel
分类号:
R723.11
DOI:
10.16840/j.issn1673-4297.2017.04.05
摘要:
目的 探讨建立脾虚肝旺型功能性消化不良(FD)幼鼠模型的方法。方法 20只SD幼鼠随机分为2组,正常组予正常饮食,模型组予不规则喂养加夹尾刺激法造模,逢双日正常进食,逢单日禁食不禁水,连续2周后,在继续上述喂养的同时,用长海绵钳夹幼鼠尾巴远端1/3处,以不破皮为度,令其暴怒,寻衅与其他幼鼠撕打,以激怒全笼幼鼠。每次刺激20 min,每隔3 h刺激1次,每日3次,连续刺激1周。观察幼鼠的一般情况及饮水量、食量、体质量变化,测定胃肠排空率,并与正常组作对照。结果 模型组幼鼠摄食量、饮水量减少,体质量减轻,与正常组比较差异有统计学意义(P<0.01);胃肠排空率下降,与正常组比较差异有统计学意义(P<0.01)。结论 不规则喂养加夹尾刺激法制造的脾虚肝旺型(FD)幼鼠模型,符合FD动物模型的生物学特征,可用于实验研究。
Abstract:
Objective To explore the model of young rats with functional dyspepsia of spleen deficiency and liver sufficiency.Methods 20 SD young rats were randomly divided into two groups.The normal group was given normal diet,while the model group was given irregular feeding and tail-clamping method.Normal feeding for young rats of model group in even-numbered days,but abrosia and normal water in odd-numbered days,which lasted for 2 weeks.At the same time,clamp their tails without skin-broken to make them be in violent rage to fighting with others,which leaded to be in rage of all young rats.This kind of stimulation lasted for 20 minutes each time,once in every three hours,three times a day.After one week,observed water and food intake,weight,rate of gastrointestinal emptying,and then compare with rats of normal group.Results Water and food intake,weight of young rats in model group was reduced than these of normal group with significant difference (P<0.01), meanwhile,the rate of gastrointestinal emptying was lower than that of normal group with significant difference (P<0.01).Conclusion The model of young rats with functional dyspepsia of spleen deficiency and liver sufficiency made by irregular fee- ding and tail-clamping stimulation conformed to the characteristics of FD animal model,which was available in experimental studies.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-12-24。
基金项目:浙江省中医药管理局基金项目(2014ZA087)。
作者简介:周红(1988-),女,住院中医师,医学硕士,主要从事儿科临床工作。
更新日期/Last Update: 1900-01-01