[1]李玉霞,史正刚,吴丽萍.增食灵颗粒对小儿厌食症动物模型P物质及生长抑素调节作用的实验研究[J].中医儿科杂志,2019,15(03):14-17.[doi:10.16840/j.issn1673-4297.2019.03.05]
 LI Yuxia,SHI Zhenggang,WU Liping.Experimental study on the regulation effect of Zengshiling Keli (增食灵颗粒) on substance P and somatostatin in animal model of children with anorexia[J].Journal of Pediatrics of Traditional Chinese Medicine,2019,15(03):14-17.[doi:10.16840/j.issn1673-4297.2019.03.05]
点击复制

增食灵颗粒对小儿厌食症动物模型P物质及生长抑素调节作用的实验研究
分享到:

《中医儿科杂志》[ISSN:/CN:62-1176/R]

卷:
15
期数:
2019年03期
页码:
14-17
栏目:
论著
出版日期:
2019-05-25

文章信息/Info

Title:
Experimental study on the regulation effect of Zengshiling Keli (增食灵颗粒) on substance P and somatostatin in animal model of children with anorexia
作者:
李玉霞12 史正刚1 吴丽萍2
1. 甘肃中医药大学中医临床学院, 甘肃 兰州 730000;
2. 甘肃中医药大学附属医院儿科, 甘肃 兰州 730020
Author(s):
LI Yuxia12 SHI Zhenggang1 WU Liping2
1. Clinical College of TCM, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
2. Department of Pediatrics, Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, Gansu, 730020, China
关键词:
厌食症小儿增食灵颗粒P物质生长抑素实验研究
Keywords:
anorexiachildrenZengshiling Keli (ZSLK)substance P (SP)somatostatin(SS)experimental study
分类号:
R965.1
DOI:
10.16840/j.issn1673-4297.2019.03.05
摘要:
目的 通过观察增食灵颗粒对小儿厌食症小鼠血浆中P物质(SP)及生长抑素(SS)水平的影响,探讨其治疗小儿厌食症的作用机制。方法 将60只昆明种幼龄小鼠随机分为空白组、模型组、增食灵颗粒小剂量组(简称小剂量组)、增食灵颗粒大剂量组(简称大剂量组)、阳性对照组。采用病因模拟法建立小儿厌食症动物模型,各组小鼠分别灌服相应药物或蒸馏水,连续灌胃10 d,分别检测小鼠血浆中SP及SS水平。结果 造模第14天,各组造模小鼠摄食量与空白组比较均显著下降(P<0.05),平均降低21.8%;至第35天,各组造模小鼠摄食量与空白组比较显著下降,差异有统计学意义(P<0.01),平均降低24.4%;各组小鼠不伴有腹泻、毛枯、少动等其他症状,说明造模成功。第40天,各组小鼠摄食量与模型组比较,差异有统计学意义(P<0.01);与阳性对照组比较,大、小剂量组摄食量无统计学意义(P>0.05)。与空白组比较,模型组SP、SS水平显著下降或升高(P<0.01);与模型组比较,大、小剂量组和阳性对照组均可显著增加SP水平或降低SS水平(P<0.05或P<0.01);大、小剂量组和阳性对照组之间两两比较,差异无统计学意义(P>0.05)。结论 增食灵颗粒可明显增加厌食症小鼠的摄食量,并能显著提高血浆SP水平,降低SS水平。
Abstract:
Objective By observing the effect of Zengshiling Keli(增食灵颗粒,ZSLK)on the plasma levels of substance P (SP) and somatostatin (SS) in the model of young Kunming mice with infantile anorexia,its mechanism of action in the treatment of infantile anorexia was discussed.Methods Sixty young Kunming mice were randomly divided into blank group,model group,ZSLK small dose group (short for small dose group),ZSLK large dose group (short for large dose group),and positive control group.The animal model of infantile anorexia was established by etiological simulation, mice in each group were administrated with corresponding drugs or distilled water,consecutive intragastric administration for 10 days,and the levels of SP and SS in plasma of mice were detected.Results On the 14th day,the food intake of the model mice in each group decreased significantly compared with that of the blank group (P<0.05),with an average decrease of 21.8%.On the 35th day, the food intake of the model mice in each group was decreased significantly than that of the blank group (P<0.01),with an average decrease of 24.4%.There were no other symptoms such as diarrhea,hair dryness and hypokinesia in each group,which indicated that the model was successfully established.On the 40th day,there was statistically significant difference between the model group and the other groups in the food intake of the mice (P<0.01);there was no significant difference between the positive control group and the small dose group and the large dose group in the food intake of the mice (P>0.05).The levels of SP and SS in the model group decreased or increased significantly compared with the blank group (P<0.01).Compared with the model group, the levels of SP and SS in the large,small dose groups and the positive control groups were significantly increased or decreased (P<0.05 or P<0.01);there was no statistically significant difference between the large,small dose groups and the positive control groups (P>0.05).Conclusion ZSLK can significantly increase the food intake of anorexic mice,and significantly increase the plasma SP level and reduce the SS level.

参考文献/References:

[1] 姜永红,孙远岭.儿童厌食与食欲调节因子的研究[J].国外医学·妇幼保健分册,2005,16(4):214-216.
[2] 史正刚,李玉霞,刘婷.增食灵颗粒对小儿厌食症动物模型瘦素的影响[J].中医儿科杂志,2009,5(2):39-40.
[3] 史正刚,芦红伟.小儿增食灵颗粒对幼龄大鼠离体小肠平滑肌的影响[J].中医儿科杂志,2007,3(2):23-24.
[4] 马蕾,刘桃安,刘恩远."小儿增食灵"对小儿厌食症动物模型下丘脑β-EP的影响[J].地方病通报,2009,24(6):28-30.
[5] 吴丽萍,任耀全,王艳丽.小儿增食灵合剂对小儿厌食症大鼠促胃液素水平的影响[J].中国中西医结合消化杂志,2010,18(5):297-299.
[6] 万力生,汪受传.儿宝颗粒对幼龄厌食大鼠胃黏膜细胞内线粒体、酶原颗粒干预的研究[J].中医儿科杂志,2006,2(1):17-20.
[7] 王倩,范文涛,周永学.硝菔通结颗粒对老年便秘大鼠肠组织P物质、血管活性肠肽含量的影响[J].陕西中医学院学报,2007,30(3):38-39.
[8] 张洪泉,许正新,周成华,等.复方大黄合剂对小鼠胃肠蠕动的影响及相关机制[J].安徽医药,2003,7(6):428.
[9] 李倩,孙远岭.小儿厌食与食欲调节研究进展[J].中国当代儿科杂志,2010,12(1):78-81.
[10] 刘淑娥.儿食健宝颗粒对幼龄厌食大鼠模型空肠段SP,VIP,SS表达影响的实验研究[J].河北医学,2007,13(12):1217-1219.

相似文献/References:

[1]辛晶.刘振寰教授治疗小儿屏气发作经验[J].中医儿科杂志,2015,(04):3.
 XIN Jing.Professor LIU Zhenhuan’s experience in treating infantile breath holding spell[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):3.
[2]潘馨,邵梦洁.穴位敷贴防治小儿感染后气道高反应咳嗽110例临床观察[J].中医儿科杂志,2015,(04):16.
 PAN Xin,SHAO Mengjie.Clinical observation of acupoint sticking in the treatment of 110 cases of children airway hyper reactivity cough after infection[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):16.
[3]谢敏,吕旭艳.黄芪颗粒治疗小儿反复呼吸道感染(营卫失和型)65例临床疗效分析[J].中医儿科杂志,2015,(04):20.
 XIE Min,LUE Xuyan.Clinical efficacy of Astragalus Particles in treating children recurrent respiratory tract infection of ying-wei disharmony type[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):20.
[4]江春燕,李成国,姚伟光.葶苈大枣泻肺汤加味外敷治疗小儿痰湿咳嗽200例临床观察[J].中医儿科杂志,2015,(04):28.
 JIANG Chunyan,LI Chengguo,YAO Weiguang.Clinical observation of external application of modified Tingli Dazao Xiefei Tang in the treatment of 200 cases of children with phlegm damp cough[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):28.
[5]汪永峰.中西医结合治疗小儿手足口病54例临床观察[J].中医儿科杂志,2015,(04):44.
 WANG Yongfeng.Clinical observation of integrated western and TCM medicine in treating 54 cases of children hand-foot-mouth disease[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):44.
[6]荣志云,张源,丛丽.小儿积滞发病的相关因素调查[J].中医儿科杂志,2015,(04):47.
 Rongzhiyun,Zhangyuan,Cong Li.Investigation on related factors of the pathogenesis of infantile dyspepsia[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):47.
[7]王良军.中西医结合治疗小儿幽门螺杆菌相关性胃炎73例临床观察[J].中医儿科杂志,2015,(04):53.
 WANG Liangjun.Clinical observation of TCM combined with western medicines in treating 73 cases of children Hp-associated gastritis[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):53.
[8]张梦桃,张映芬,李玉秀,等.脑瘫患儿睡眠障碍的护理[J].中医儿科杂志,2015,(04):70.
 ZHANG Mengtao,ZHANG Yingfen,LI Yuxiu,et al.Nursing care on sleep disorders of children with cerebral palsy[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):70.
[9]邬颖菲.苍苓止泻口服液辅助治疗小儿脾虚泻的临床疗效及护理措施[J].中医儿科杂志,2015,(04):73.
 WU Yingfei.Clinical efficacy and nursing care in the adjuvant treatment of spleen deficiency diarrhea in children with Cangling Zhixie Koufuye[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):73.
[10]张春玲.倪晓红主任医师论治小儿疳积经验[J].中医儿科杂志,2015,(02):10.
 ZHANG Chunling.Chief physician NI Xiaohong’s experience on the treatment of infantile malnutrition[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):10.
[11]钱雁.自拟疏肝运脾汤治疗小儿厌食症42例临床观察[J].中医儿科杂志,2015,(05):36.
 QIAN Yan.Clinical observation of homemade Shugan Yunpi Tang in treating 42 cases of pediatric anorexia[J].Journal of Pediatrics of Traditional Chinese Medicine,2015,(03):36.
[12]唐芳芳,张春霖,韦美宾,等.壮医针挑疗法对脑瘫厌食症患儿的疗效及血清微量元素的影响[J].中医儿科杂志,2016,(02):57.[doi:10.16840/j.issn1673-4297.2016.02.19]
 TANG Fangfang,ZHANG Chunlin,WEI Meibin,et al.Effect of Zhuang medicine acupuncture on blood trace elements in anorexia children with cerebral palsy[J].Journal of Pediatrics of Traditional Chinese Medicine,2016,(03):57.[doi:10.16840/j.issn1673-4297.2016.02.19]
[13]陶奕红.健胃消食口服液治疗小儿厌食症脾胃虚弱型66例临床观察[J].中医儿科杂志,2016,(03):43.[doi:10.16840/j.issn1673-4297.2016.03.17]
 TAO Yihong.Efficacy of Jianwei Xiaoshi Oral Liquid in the treatment of infantile anorexia due to weakness of spleen and stomach[J].Journal of Pediatrics of Traditional Chinese Medicine,2016,(03):43.[doi:10.16840/j.issn1673-4297.2016.03.17]
[14]王霞芳.小儿厌食症的辨证分型论治[J].中医儿科杂志,2007,(04):28.
[15]石艳红,肖达民.小儿厌食症的中医治疗概况[J].中医儿科杂志,2007,(06):43.
[16]方娜丽,何建平,张平发,等.参术儿康糖浆治疗小儿厌食症60例疗效观察[J].中医儿科杂志,2008,(02):30.
[17]何雯,李家凤,张瑛.健脾剂治疗小儿厌食症50例临床观察[J].中医儿科杂志,2009,(04):15.
 He Wen,Li Jiafeng,Zhang Ying.Jianpi Prescription for children anorexia: an observation of 50 cases[J].Journal of Pediatrics of Traditional Chinese Medicine,2009,(03):15.
[18]吴淑莲.中西医结合治疗小儿厌食症80例疗效观察[J].中医儿科杂志,2009,(05):29.
 Wu Shulian.TCM combined with Western medicine for children anorexia:an observation of 80 cases[J].Journal of Pediatrics of Traditional Chinese Medicine,2009,(03):29.
[19]徐曼曼,许洪义,任光明.健儿开胃散治疗小儿厌食症肝旺脾虚证临床研究[J].中医儿科杂志,2013,(02):28.
 Xu Manman,Xu Hongyi,Ren Guangming.Clinical research of Jianerkaiwei Powder in treating infantile anorexia of spleen deficiency and liver hyperactivity[J].Journal of Pediatrics of Traditional Chinese Medicine,2013,(03):28.
[20]樊彩娥.运脾喜食方治疗小儿厌食症60例疗效观察[J].中医儿科杂志,2013,(04):27.
 Fan Caie.Curative effect observation of 60 cases of infantile anorexia treated with Yunpixishi Decoction[J].Journal of Pediatrics of Traditional Chinese Medicine,2013,(03):27.

备注/Memo

备注/Memo:
作者简介:李玉霞(1979-),女,副主任医师,在读博士研究生。研究方向:中医药防治小儿脾胃及心肝疾病的研究。
通信作者:史正刚(1963-),男,主任医师,教授,博士生导师,医学博士,主要从事中医儿科临床、教学及科研工作。E-mail:szg@gszy.edu.cn
更新日期/Last Update: 1900-01-01